Alcoholic formalin is the ideal fixative for laboratories in colder environments or for samples in freezer storage. It is used to promote rapid penetration and coagulant fixation without lysis of erythrocytes, and its neutral pH between 6.8 and 7.2 promotes the preservation of delicate tissue structures.
Our B-5 fixative is made in the traditional formulation containing mercuric chloride. It is considered the gold standard for immunohistology testing, particularly for detailed analysis of bone marrow, tissues of the spleen, and lymph nodes when lymphomas are suspected. This product comes in a kit containing one pre-measured vial of B-5 fixation and one vial of 37% formaldehyde to be mixed thoroughly before application.
Bouin’s solution is a versatile fixative that contains bright yellow pigmentation properties. It is a popular choice for fixing embryonic studies and skin, due to its excellent preservation and illumination of protoplasmic elements such as nuclei and chromosomes. It may be used to decalcify bones with the addition of formic acid, a combination that is delicate enough to preserve the boney tissue for several days or weeks. It may also be used to for adipose rich tissues when 25% ethanol is added.
Buffered zinc formalin is often used as a safer substitute for B-5 on tissue samples of the spleen, bone marrow, and breast. It is compatible with a wide range of staining agents, and its zinc chloride compound increases the rigidity of the cellular elements as to prevent brittle sectioning during staining and processing procedures. For best results, first fix the specimen with NBF.
Carson’s modified millonig’s is a buffered fixative often used to prepare a wide range of tissue samples for light or electron microscopy. It may be used in these routine fixations as opposed to NBF, while similarly promoting rapid tissue penetration, excellent cellular detail, and ultrastructure preservation for later examination.
Cytospin fluid is formulated with a mixture of alcohol and carbo-wax. This combination helps to maintain and enhance cellular embedded in fixated tissue, as well as guard it against airdrying artifacts. It is routinely used to fix aspirated cells and those obtained through urine and bronchial washings. An application of cytospin fluid will store the given sample for weeks without any adverse effects.
Decalcifier is predominantly used to remove calcium and minerals on bone marrow tissues in preparation for the embedding of a fixation agent. Fixation time will vary based on the size of the specimen undergoing an application, with the larger, denser bones requiring much longer decalcifying times.
Fix-d-cal is an original Kyron Sciences formalin that acts decalcifier as well as a tissue fixative for boney tissues. The formic acid and sodium citrate are used to gently dissolve the boney elements of the tissue while simultaneously retaining excellent soft tissue morphology. Then, its formalin component provides detailed fixation without a secondary additive.
Formalin neutralizer is used to neutralize toxic chemicals in buffered fixatives, both depleting the risk of formaldehyde-based fluids/vapors and making them safe for drain disposal. Neutralization time varies between a few minutes to a few hours based upon the volume of the applicable waste.
4 x 0.5gal, 2 liter neutralizes 18 gallons formalin.
Hartman’s fixative is often used as a safer and more efficient alternative to bouin’s fixative. Comparatively, it produces less shrinkage and superior morphologic detail after application. It may be used in the preservation of eye tissue, bone marrow, fatty tissues from the breast and testis, as well as others for histological examination. Its versatility also makes it effective in the location of lymph nodes in radical dissection specimens, causing the glands to distinctively turn white.
Hollande’s fixative is an excellent multi-purpose formalin providing numerous application benefits. Its cupric acetate component stabilizes red cell membranes, as well as eosinophils and endocrine cells in tissue samples as protection measures against lysis. It is often used in gastrointestinal and prostrate biopsies, but may also be used on bone marrow and any tissue containing a large amount of inflammatory or muscosa cells. For firmer tissue, Hollande’s fixative softens the structure to avoid brittle sectioning during microscopic examination.
10% neutral buffered formalin (nbf) is considered the standard fixative for histology tissues examined by light microscopy. It is a convenient option for a wide range of samples in that it provides a short-fixation time, long preservation, and its thick consistency has the ability to delicately preserve cellular structures.
Paraformaldehyde (PFA) is a multi-use solution. It is commonly used for research purposes, but may also be used to fix nervous and cardiovascular tissues after it has been depolymerized in a formaldehyde-based solution. It contains a neutral pH level between 6.8 and 7.2, ensuring that tissue applications retain their protoplasmic structures and details for microscopic examination. Our paraformaldehyde is unique in that it contains two aldehydes per molecule thus providing a stronger fixation.
Saccomanno fluid contains alcohol and carbo-wax which work in tandem to illuminate protoplasmic details, prevent air drying artifacts from obstructing fixated tissue samples, and provide long-term storage capabilities up to several days to weeks. It acts as a versatile cytology fixative often used on samples obtained through urine samples and bronchial washings. Other feasible surfaces include lung tissue, sputum specimen, and pleural and peritoneal fluids.
This product includes one pre-measured vial of SAF fixative and one tube that may be used store stool samples. When applied, ova and parasites are detected even when present in very low numbers. SAF formalin is a simple yet convenient fixation agent, in that it is suitable for the examination, concentration, permanent staining, and preservation of a singular sample. This product does not contain mercuric chloride.
Fecal Transport tube w/ 15ml SAF Fixative 2 pk 200/cs
This product includes one pre-measured vial of mercury PVA and one tube that may be used store stool samples. When applied, ova and parasites are detected even when present in very low numbers. Mercury PVA is widely considered the “gold standard” fixative agent for fecal samples as its mercuric compounds harbor strong pigmentation and stabilizing properties.
PVA & Formalin fecal transport tubes 100 Sets/ cs
This product includes one pre-measured vial of modified PVA and one tube that may be used store stool samples. When applied, ova and parasites are detected even when present in very low numbers. Modified PVA is formulated with copper sulfate as opposed to mercuric chloride, making it a safer solution to work with during fixation and disposal processes. It is specifically effective in the fixation and preservation of protozoan trophozoites when stains are added (typically trichrome or hematoxylin).
This product includes one pre-measured vial of zinc PVA formalin and one tube that may be used store stool samples. When applied, ova and parasites are detected even in very low numbers. It presents itself as a safer alternative to mercury PVA in that it lacks mercuric compounds, but similarly contains strong pigmentation properties and morphologic preservation abilities.
Zinc PVA and Formalin fecal transport tube 100 sets/cs